Comparative proteomic analysis reveals different responses in porcine lymph nodes to virulent and attenuated homologous African swine fever virus strains

Herrera-Uribe, Júber; Jiménez-Marín, Ángeles; Lacasta, Anna; Monteagudo, Paula L.; Pina-Pedrero, Sonia; Rodríguez, Fernando; Moreno, Ángela; Garrido, Juan J.

doi:10.1186/s13567-018-0585-z

Veterinary Research

Table 2 The top proteins with the largest change in expression in response to ASFV infection (1 dpi)

From: Comparative proteomic analysis reveals different responses in porcine lymph nodes to virulent and attenuated homologous African swine fever virus strains

							Attenuated-control
ID SSP	ID	Gen name	Protein name	pI	Mw (kDa)	% coverage	Fold change	p value
5605	F1RUN2	ALB	Serum albumin Sus scrofa	5.98	71.60	19	34.7	0.0006
605	P02543	VIM	Vimentin Sus scrofa	5.06	53.70	63	27.5	0.001
1307	Q6QAQ1	ACTB	Actin.cytoplasmic 1 Sus scrofa	5.29	42.1	19	11	0.0002
6718	P02554	TUBB	Tubulin beta chain Sus scrofa	4.78	50.30	17	10.1	0.0024
8411	I7GKE9	EEF2	Similar to human eukaryotic translation elongation factor Macaca fascicularis	5.93	36.4	21	6.7	0.002
4807	F1RMN7	HPX	Hemopexin Sus scrofa	6.59	52.10	21	4.5	0.0003
8315	F1RS36	HSPA5	78 kDaglucose-regulated protein Sus scrofa	5.21	70.3	24	4.2	0.0175
8219	I3L816	HNRNPH1	Heterogeneous nuclear ribonucleoprotein H Sus scrofa	6.44	46.6	34	3.8	0.0014
8107	K7EJP1	ATP5A1	ATP synthase subunit alpha.mitochondrial Homo sapiens	5.51	15.40	29	3.5	0.0047
9602	I3LK59	ENO	Enolase Sus scrofa	8.93	38.20	29	3.4	0.0005
4806	Q6S4N2	HSPA1B	Heat shock 70 kDa protein 1B Sus scrofa	5.6	70.30	18	− 23.1	0
9508	I3LEC2	PCBP1	Poly(rC)-binding protein 1 Sus scrofa	6.66	37.9	39	− 15	0.0001
7420	F1RKU0	IDH3A	Isocitrate dehydrogenase [NAD] subuni talpha, mitochondrial Sus scrofa	6.72	40.1	24	− 10	0.0105
407	F1M0S3	TPM2	Tropomyosin beta chain Rattus norvegicus	5.19	31.1	38	− 9.6	0.0003
502	C9J9K3	RPSA	40S ribosomal protein SA Homo sapiens	5.15	30	62	− 8.6	0.0001
302	P62258	YWHAE	14-3-3 protein epsilon Homo sapiens	4.79	29.2	44	− 7.5	0.0023
9804	P09571	TF	Serotransferrin Sus scrofa	6.93	78.90	36	− 6.9	0.0013
9401	P00355	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase Sus scrofa	8.51	36.1	34	− 5.7	0.001
205	F2Z558	YWHAZ	14-3-3 protein zeta Sus scrofa	4.77	28.20	42	− 5.4	0.0032
9107	F1S3U9	PRDX1	Peroxiredoxin 1 Sus scrofa	8.67	22.1	44	− 5.1	0.0052

							Virulent-control
ID SSP	ID	Gen name	Protein name	pI	Mw (kDa)	% coverage	Fold change	p value
4107	F1RUN2	ALB	Serum albumin Sus scrofa	5.98	71.60	5	13.4	0.0012
6718	P02554	TUBB	Tubulin beta chain Susscrofa	4.78	50.30	17	11.3	0.0003
5411	F1RPH0	PGK1	Phosphoglycerate kinase Sus scrofa	6.32	43.4	30	7.3	0.0009
8214	D0G7F6	TPI1	Triosephosphate isomerase Sus scrofa	6.54	23.90	89	6.5	0.001
8411	I7GKE9	EEF2	Similar to human eukaryotic translation elongation factor Macaca fascicularis	5.93	36.4	21	6.5	0.0023
4308	B6VNT8	ACTC1	Alpha actin 1 Sus scrofa	5.23	42.3	8	5	0.0005
8107	K7EJP1	ATP5A1	ATP synthase subunit alpha.mitochondrial Homo sapiens	5.51	15.40	29	3.5	0.0002
8219	I3L816	HNRNPH1	Heterogeneous nuclear ribonucleoprotein H Sus scrofa	6.44	46.6	34	3.4	0.0008
1410	Q9N0Y9\|	TMOD3	Ubiquitous tropomodulin U-Tmod Sus scrofa	4.98	39.7	31	2.4	0.0002
7504	F1SVB0	CAPG	Macrophage-capping protein Sus scrofa	5.88	39.2	23	2.3	0.0022
9107	F1S3U9	PRDX1	Peroxiredoxin 1 Sus scrofa	8.67	22.1	44	− 6.1	0.0009
302	P62258	YWHAE	14-3-3 protein epsilon Homo sapiens	4.79	29.2	44	− 13.6	0.0027
8806	P09571	TF	Serotransferrin Sus scrofa	6.93	78.9	32	− 11.7	0.0001
301	Q5ISS9	YWHAQ	14-3-3 protein theta isoform Sus scrofa	4.7	29.20	49	− 11.4	0.0002
7715	F1RFN9	FSCN1	Fascin Sus scrofa	6.04	55.20	26	− 9.8	0.001
8404	A2A6G8	Lasp1	LIM and SH3 domain protein 1 Mus musculus	9.14	12.1	50	− 7.5	0.0004
8510	I3LDC7\|	IDH1	Isocitrate dehydrogenase [NADP] (Fragment) Sus scrofa	7.64	48.7	24	− 7	0.0011
407	F1M0S3	TPM2	Tropomyosin beta chain Rattus norvegicus	5.19	31.1	38	− 5.2	0.0007
502	C9J9K3	RPSA	40S ribosomal protein SA Homo sapiens	5.15	30	62	− 4.8	0.0002
5113	Q08024	CBFB	Core-binding factor subunit beta Mus musculus	5.59	22.2	31	− 4.4	0.0008

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