Skip to main content

Table 1 Strains, plasmids, and primers used in this study

From: The Riemerella anatipestifer M949_RS01035 gene is involved in bacterial lipopolysaccharide biosynthesis

Strains, plasmids or primers

Characteristics

Source or references

Strains

 CH3

Riemerella anatipestifer serotype 1 strain

[6]

 RA1062

Tn4351 insertion mutant of Riemerella. anatipestifer CH3, M949_RS01035:: Tn

This study

 BW19851(pEP4351)

Plasmid pEP4351 in BW19851, CmR

[6]

 WJ4

R. anatipestifer WT strain, serotype 1

[6]

 Yb2

R. anatipestifer WT strain, serotype 2

[6]

 HXb2

R. anatipestifer WT strain, serotype 10

[6]

Primers

 16S rRNA F

5′-GAGCGGTAGAGTATCTTCGGATACT-3′

This study

 16S rRNA R

5′-AATTCCTTTGAGTTTCAACCTTGCG-3′

This study

 TN-1

5′-GGACCTACCTCATAG-3′

This study

 IS4351-F

5′-TCAGAGTGAGAGAAAGGG-3′

This study

 Tn4351-F

5′-TGGCACCTTTGTGGTTCTTAC-3′

This study

 Tn4351-R

5′-GAGAGACAATGTCCCCCTTTC-3′

This study

 Erm-F

5′-GCCCGAAATGTTCAAGTTGT-3′

This study

 Erm-R

5′-CTTGACAACCACCCGACTTT-3′

This study

 M949_RS10475-F

5′-CCAAACCATATGAACCATCCTGT-3′

This study

 M949_RS10475-R

5′-GCATTATCTTCTGACAGGAGAGG-3′

This study

 M949_RS01035F

5′-TATAAAGCCTACAATAGC-3′

This study

 M949_RS01035R

5′-ATTAATTGAAGAGTTTGC-3′

This study

 M949_RS01030-F

5′-TCAATTGCTGAATCCAAACGC-3′

This study

 M949_RS01030-R

5′-TTCAGGCATTGTTGTGATGTC-3′

This study

 RA ldh-F

5′-AGAGGAGCTTATCGGCATCA-3′

This study

 RA ldh-R

5′-CTAGGGCTTCTGCCAATCTG-3′

This study