Skip to main content
Figure 4 | Veterinary Research

Figure 4

From: Salmonella Gallinarum delivering M2eCD40L in protein and DNA formats acts as a bivalent vaccine against fowl typhoid and H9N2 infection in chickens

Figure 4

In vitro proliferations of lymphocytes from vaccinated chickens in response to recall M2e or ompA antigen. Chickens (N = 10) were vaccinated with JOL916, JOL2068, JOL2074 or JOL2074 + JOL2076, and PBMCs from vaccinated chickens after 14 days post-immunization were restimulated with either M2e or ompA (10 µg/mL) for 72 h and lymphocyte proliferation was determined by MTT-based assay. Results are expressed as stimulation indices, defined as proliferations in response to recall antigen relative to the mock stimulated cells. A M2e-specific lymphocyte proliferation assay. B ompA-specific lymphocyte proliferation assay. Each data points represent mean ± SD of five chickens per group. Statistical significance was determined by one-way ANOVA (with Tukey’s multiple comparisons tests). *p < 0.05. ns: non-significant.

Back to article page