Figure 4

Inhibition of Salmonella Typhimurium motility by methyl gallate alone and in combination with a sub-minimum inhibitory concentration (MIC) of marbofloxacin. A Inhibition of Salmonella motility on semi-solid LB plate, bacteria were inoculated at the center of the LB plate containing 0.3% agar and incubated for 13 h at 37 °C. (a) Salmonella Typhimurium inoculated in LB plate containing no antibiotic (b) plate containing sub-MIC of MRB and inoculated with Salmonella Typhimurium, (c) plate containing the combination of MG (30 μg/mL) with sub-MIC of MRB and inoculated with Salmonella Typhimurium, (d) plate containing 30 µg/mL of MG and inoculated with Salmonella Typhimurium, and (e) plate containing 100 µg/mL of MG and inoculated with Salmonella Typhimurium. B Inhibition of Salmonella Typhimurium motility in three different strains by MG. Images are representatives of three different experiments. The control comprises Salmonella Typhimurium cultured on agar plates without any drugs; sub-MIC refers agar plate containing sub-MIC of MRB; MG-30 refers to treated with 30 µg/mL of methyl gallate alone; MGM refers to the treatment with a combination of methyl gallate (30 µg/mL) and sub-MIC of MRB; MG-100 refers to treated with 100 µg/mL of methyl gallate.