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Table 2 Vaccination schedule

From: Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses

Group

Horses

Vaccine

Protein content

Dose and route of administration

1

Horse 1

Horse 2

Gradient purified AHS 5 VLPs

AHSV 5 VP2, VP3, VP5 and VP7

± 200 μg total protein in 5 mL PBS pH 7.4 with 5% Pet Gel A adjuvant, intra-muscular

2

Horse 3

Horse 4

Filtered AHS 5 VLPs

AHSV 5 VP2, VP3, VP5 and VP7

± 100 μg total protein in 5 mL bicine buffer pH 8.5 with 5% Pet Gel A adjuvant, intra-muscular

3

Horse 5

Horse 6

PBS

Nil

5 mL PBS pH 7.4 with 5% Pet Gel A adjuvant, intra-muscular

4

Horse 7

Horse 8

Bicine

Nil

5 mL bicine buffer pH 8.5 with 5% Pet Gel A adjuvant, intra-muscular

  1. Horses were vaccinated with either gradient-purified (Group 1) or filtered (Group 2) plant-produced AHS 5 VLPs, or PBS (Group 3) or bicine buffer (Group 4), all adjuvanted with 5% Pet Gel A (SEPPIC, Paris, France) and administered by deep intra-muscular injection.