From: Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses
Group | Horses | Vaccine | Protein content | Dose and route of administration |
---|---|---|---|---|
1 | Horse 1 Horse 2 | Gradient purified AHS 5 VLPs | AHSV 5 VP2, VP3, VP5 and VP7 | ± 200 μg total protein in 5 mL PBS pH 7.4 with 5% Pet Gel A adjuvant, intra-muscular |
2 | Horse 3 Horse 4 | Filtered AHS 5 VLPs | AHSV 5 VP2, VP3, VP5 and VP7 | ± 100 μg total protein in 5 mL bicine buffer pH 8.5 with 5% Pet Gel A adjuvant, intra-muscular |
3 | Horse 5 Horse 6 | PBS | Nil | 5 mL PBS pH 7.4 with 5% Pet Gel A adjuvant, intra-muscular |
4 | Horse 7 Horse 8 | Bicine | Nil | 5 mL bicine buffer pH 8.5 with 5% Pet Gel A adjuvant, intra-muscular |