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Table 3 Spiking experiment results indicating the efficiency of Treponema DNA extraction and detection, with and without bovine tissue, using species-specific qPCR

From: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species

Species

1

Mean counta

1/10

Mean count

1/100

Mean count

Average efficiency (%)

Culture

Tissue (count/mg)

Culture

Tissue (count/mg)

Culture

Tissue (count/mg)

T. phagedenis

2.32 × 108

4.22 × 107

2.78 × 108

3.32 × 107

2.26 × 107

1.04 × 107

99.5

T. medium

8.72 × 107

9.76 × 105

1.40 × 108

6.80 × 106

1.22 × 107

9.45 × 105

98.8

T. pedis

1.41 × 109

7.93 × 107

6.85 × 105

7.80 × 104

9.31 × 104

5.25 × 103

96.0

T. denticola

2.58 × 106

1.24 × 105

4.14 × 105

8.36 × 104

1.23 × 105

1.59 × 104

98.2

  1. aCq values were converted into gene copy numbers and the mean count between samples without tissue was compared to the mean count with tissue samples of tenfold serially diluted Treponema cultures.
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Veterinary Research

ISSN: 1297-9716