Figure 2
From: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2
S. suis is unable to cleave human IgA1. Purified recombinant Zmp protein (100 µg), washed bacteria (bact) or bacterial supernatant (sup) from S. suis (SS) or S. pneumoniae (SP) were incubated with human IgA1 for 16 h and reactions separated by SDS-PAGE. Cleaved IgA1 (red arrow) by washed S. pneumoniae bacteria or bacterial supernatant (lanes 3 and 4) and uncleaved IgA1 (black arrow) by washed S. suis bacteria (lane 5), bacterial supernatant (10X, lane 6), and purified Zmp protein (lane 7) were visualized using a specific antibody against human IgA1. MW corresponds to the molecular weight ladder (lane 1) and media correspond to untreated human IgA1 (lane 2).