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Figure 4 | Veterinary Research

Figure 4

From: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2

Figure 4

S. suis is unable to cleave P-selectin glycoprotein ligand-1 (PSGL-1) ectodomains. Purified recombinant Zmp protein (100 µg), washed bacteria (bact) or bacterial supernatant (sup) from S. suis (SS) or S. pneumoniae (SP) were incubated with PSGL-1/fc for 1 h. Samples were separated by SDS-PAGE and transferred onto PVDF membranes, which were incubated with KPL-1 (A) or PL2 (B) antibody. No recognition of PSGL-1 by the KPL-1 antibody and visualization of lower molecular weight band with PL2 antibody were observed for washed S. pneumoniae bacteria and bacterial supernatant (lanes 3 and 4), which is expected following PSGL-1 ectodomain cleavage. There was no change in the recognition by KPL-1 or in the molecular weight visualized with PL2 for PSGL-1 treated with washed S. suis bacteria (lane 5), bacterial supernatant (10X, lane 6) or the purified recombinant Zmp protein (lane 7). MW corresponds to the molecular weight ladder (lane 1) and media correspond to untreated PSGL-1 (lane 2).

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