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Table 1 The proteins with significant changes in abundance

From: Fructose-1,6-bisphosphate aldolase encoded by a core gene of Mycoplasma hyopneumoniae contributes to host cell adhesion

Spot no.

Protein description

Fold change

Mascot scorea

Theoretical pI/Mw

Sequence coverage (%)

Accession

1

Molecular chaperone DnaK (DnaK)

+

179

5.17/68281.56

6

ADQ90292.1

2

Elongation factor Tu (EF-Tu)

1.88

711

5.61/44122.49

27

ADQ90729.1

3

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH)

+

198

6.67/36873.02

11

ADQ90256.1

4

Adenine phosphoribosyltransferase (Apt)

1.94

216

5.34/18849.21

22

ADQ90402.1

5

l-Lactate dehydrogenase (LDH)

1.72

442

8.29/34237.25

20

ADQ90382.1

6

Heat shock protein (GrpE)

1.97

87

5.47/28854.10

6

ADQ90239.1

7

Fructose-bisphosphate aldolase (FBA)

+

342

9.05/43780.88

20

ADQ90241.1

  1. aMascot computes a score based on the probability that the peptides from a sample match those in the selected protein database. The more peptides Mascot identifies from a particular protein, the higher the Mascot score for that protein.
  2. “+” Means those proteins that only observed in the 2-DE map of M. hyopneumoniae strain 168, which means its expression level in strain 168L is very low.