Figure 3

RT-QuIC data from sCJD-infected, uninfected and indeterminate CWD-inoculated tg66 mice. Additional RT-QuIC analysis was performed on four CWD-inoculated mice that gave positive amyloid seeding activity results on our initial screening runs to assess reproducibility of the data. Each point represents the normalized fluorescence value for an individual well measured at 25 h of RT-QuIC reaction time. Results from 3 to 4 independent assays testing 4–16 wells per mouse per assay have been pooled. The total number of wells tested for each mouse and the number of positive and negative control wells present on the same trays is provided below the x-axis. Statistical analysis was performed comparing data from individual mice to the uninfected control mice using Fisher’s exact test, *** indicates p < 0.0001, ** indicates p = 0.0013.