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Figure 4 | Veterinary Research

Figure 4

From: Chicken cathelicidin-2 promotes NLRP3 inflammasome activation in macrophages

Figure 4

NEK7 is involved in CATH-2-induced NLRP3 activation in LPS-primed macrophages. Cells were transfected with NEK7 siRNA and control siRNA for 24 h. Subsequently, cells were primed with LPS for 3 h and then CATH-2 (5 μM) were added for an additional 21 h. Cell supernatants and lysates were collected for different assays. ELISA was used to determine cytokine secretion of IL-1β (A) and IL-6 (B). A representative image (Western blot) is presented to show protein expression including IL-1β, caspase-1, pro-IL-1β, pro-caspase-1 and β-action (C). Data in A, B are represented as mean ± SEM from three independent experiments with triplicate samples per experiment. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.005.

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