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Figure 1 | Veterinary Research

Figure 1

From: Immortalised canine buccal epithelial cells’ CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol

Figure 1

CD49f expression and immortalisation of canine buccal epithelial cells. A CD49f and cytokeratin 3 staining of canine and porcine buccal cryosections. A CD49f antibody stains the basal side of the epithelium of the canine and porcine buccal mucosa while a cytokeratin 3 antibody only stains the porcine epithelium. Cryosections of the canine and porcine buccal mucosa were stained with CD49f or cytokeratin 3 (FITC) and counterstained with Hoechst. E: epithelium, S: subepithelial tissue. B. The SV40 large T antigen sequence in the genome of canine buccal epithelial cells. DNA extraction, PCR and agarose gel electrophoresis was performed on SV40 large T antigen-transduced and -untransduced canine buccal epithelial cells using an SV40T-specific primer pair. C. Immortalised canine buccal epithelial cells express CD49f. Immortalised canine buccal epithelial cells were stained for CD49f (green) or isotype control (red) and analysed by flow cytometry. FSC: forward scatter.

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Veterinary Research

ISSN: 1297-9716