Skip to main content
Figure 1 | Veterinary Research

Figure 1

From: Comprehensive evaluation of the safety and immunogenicity of a gene-deleted variant pseudorabies virus attenuated vaccine

Figure 1

The generation of the gene-deleted PRV GX-ΔTK/IES recombinant virus. A Schematic representation of the engineered construct. The deleted regions are indicated in the full-length genome. B No fluorescent plaque or typically large syncytia were observed in PK-15 cells infected with PRV GX-ΔTK/IES. PK-15 cells infected with the marker virus PRV GX-ΔTK/IES-EGFP exhibited significant green fluorescence. C Identification and genetic stability of the recombinant PRV GX-ΔTK/IES assessed by specific PCR. A 350 bp fragment carrying the TK gene deletion and a 250 bp fragment carrying the gI/gE/US9/US2 gene deletion were amplified. Comparable DNA fragments were analysed from passage levels 1, 5, 15, and 20. PRV GX was used as the control without deletions. D IF analysis of PK15 cells infected with PRV GX-ΔTK/IES. The cells were stained using PRV gB- and gE-specific mAbs. The infected cell culture was anti-gB positive when treated with the PRV GX-ΔTK/IES and parental PRV GX, while the cultures were anti-gE negative when treated with the PRV GX-ΔTK/IES but anti-gE positive when treated with parental PRV GX. Control cells remained negative.

Back to article page