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Figure 3 | Veterinary Research

Figure 3

From: Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis

Figure 3

CUR promotes polarization and reverses PRV-induced mitochondrial dysfunction in PRV-infected BV2 cells. BV2 cells were infected with/without PRV for 24 h and then treated with/without 20 μM CUR for 24 h. A The expression of the M1 phenotype surface markers CD40 and CD16/32 and B the M2 phenotype surface markers CD206 and ARG-1 in BV2 cells was determined using flow cytometry (n = 3). C Mitochondrial structures in BV2 cells were observed using transmission electron microscopy (n = 3); scale bar = 500 nm. D Mitochondrial membrane potential (MMP) in BV2 cells. E MMP in BV2 cells was observed using fluorescence microscopy (n = 4); scale bar = 200 μm. All experiments were performed in parallel. The results are presented as the mean ± SD. Statistical significance was determined using one-way ANOVA followed by an LSD post hoc test for multiple comparisons among the groups. *P < 0.05, **P < 0.01, ***P < 0.001, and NS, not significant.

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