Figure 5

Distinct mRNA signatures were identified during phenotypic transformation according to RNA-Seq analysis. BV2 cells were infected with/without PRV for 24 h and then treated with/without 20 μM CUR for 24 h for RNA sequencing and analysis. Differentially expressed genes (DEGs) were detected by RNA-Seq analysis using DESeq2 (n = 3, P < 0.05, and fold change > 1). A Venn diagram showing DEGs. B DEG volcano plot of the PRV group vs. the control group. C DEG volcano plot of the PRV + CUR group vs. the PRV group. D GO enrichment analysis was performed on the DEGs in the PRV group vs. control group. E GO enrichment analysis was performed on the DEGs in the PRV + CUR group vs. PRV group. F KEGG enrichment analysis was performed on the DEGs in the PRV group vs. control group. G KEGG enrichment analysis was performed on the DEGs in the PRV + CUR group vs. PRV group. D KEGG enrichment analysis of DEGs. H) qRT‒PCR verification of differentially expressed genes identified in RNA-seq (n = 3). All experiments were performed in parallel. The results are presented as the mean ± SD. Statistical significance was determined using one-way ANOVA followed by an LSD post hoc test for multiple comparisons among the groups. *P < 0.05, **P < 0.01, ***P < 0.001, and NS, not significant.