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Figure 3 | Veterinary Research

Figure 3

From: Guanylate-binding protein 1 inhibits nuclear delivery of pseudorabies virus by disrupting structure of actin filaments

Figure 3

Knockout of GBP1 enhances PRV infection in PK-15 cells. A Schematic diagram representation of sgRNA targeting at the GBP1 genomic region. Red arrows indicate the sgRNA-targeting sites (sites 1, 2, and 3) located on exon3, exon3 exon4. The protospacer adjacent motif (PAM) sequences are highlighted in red and the sgRNA targeting sites are in green. B Representative Western blots of GBP1. C The cell viability was measured by MTT. D Sequencing of GBP1 locus amplified from the 23PK-15GBP1-/- and 109PK-15GBP1-/- cells. Red arrows indicate deleted bases, red characters indicate deleted bases or mutated amino acids. E, F The described cells were infected with PRV (1 MOI) for 24 h. Relative change in amount of PRV copies E Representative Western blots of viral gC and GADPH (F). (G) Quantification of panel F. ** P < 0.01, versus the wild-type PK-15 cells. H Representative immunofluorescent stain of DAPI (blue) and F-actin (green). Scale bar, 20 μm.

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