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Figure 4 | Veterinary Research

Figure 4

From: Guanylate-binding protein 1 inhibits nuclear delivery of pseudorabies virus by disrupting structure of actin filaments

Figure 4

GBP1 inhibits the nuclear delivery of PRV virions requiring its GTPase activity. The wild-type GBP1(WT) and two GBP1 mutants GBP1(K51A) and GBP1(ΔCAAX) were constructed into the pCI-neo vector, subsequently stably transfected into 109PKGBP1−/− cells, respectively defined 109PKGBP1(WT), 109PKGBP1(K51A) and 109PKGBP1(ΔCAAX). A Schematic representation of full-length GBP1, two site-specific mutants GBP1. (B) Representative Western blots of GBP1 protein. C Quantification of panel B. D–E The three cell lines were infected with PRV (1 MOI) for 24 h. Relative PRV genome copies D and representative Western blots of GBP1, gC and GAPDH (E). F Quantification of panel E. G Described cells were incubated with 10 MOI PRV at 37 ℃ for 2 h. Relative PRV genome copy number in the cell nucleus. H Representative immunofluorescent stain of DAPI (blue), HA-GBP1 (cherry), and F-actin (green). Scale bar, 20 μm. ns, not significant; ** P < 0.01, versus 109PKGBP1(WT) cells.

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