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Figure 2 | Veterinary Research

Figure 2

From: Fowl adenovirus serotype 4 enters leghorn male hepatocellular cells via the clathrin-mediated endocytosis pathway

Figure 2

FAdV-4 entry into LMH cells depends on clathrin-mediated endocytosis. A Western blot analysis after the entry of FAdV-4 into chlorpromazine-treated cells. β-Actin was used as the internal control. B qPCR analysis after the entry of FAdV-4 into chlorpromazine-treated cells. C CLSM analysis of F-actin (red), anti-FAdV-4 fibre 2 (green) and DAPI (blue) in FAdV-4-infected LMH cells pretreated with chlorpromazine. D Western blot analysis of the protein expression of CHC after the entry of FAdV-4 in cells transfected with CHC shRNA and mock control vectors. E qPCR analysis after the entry of FAdV-4 into cells transfected with CHC shRNA and mock control vectors. Bars, 20 μm. *, P < 0.05; **, P < 0.01.

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Veterinary Research

ISSN: 1297-9716