Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves

Table 1 Primers used for RT-qPCR

Gene name	Gene bank accession no.	Primer sequence (5′ – 3′)	Amplicon length (bp)
bACTIN	NM_173979.3	F: AGCCTTCCTTCCTGGGCAT	93
		R: TAGAGGTCCTTGCGGATGTC
GLUT1	NM_174602.2	F: CGGCATCAACGCTGTTTTCT	105
		R: GAAGGCTGTGTTGACGATGC
GLUT2	XM_005201668.4	F: GGTTCATGGTGGCTGAGTTT	161
		R: AGACCACACCAGCAAAAAGG
HK1	NM_001012668.2	F: AGCTTCATCCGCACTTCTCC	96
		R: CCGCTGCCGTCTTCAGATAA
HK2	XM_015473383.2	F: ATGGGCATGAAGGGTGTGTC	89
		R: CTTCAGGAGGATGCTCTCGTC
PFKl	NM_001080244.2	F: ATCGTCATGTGCGTCATCCC	110
		R: GATGCGGTCACAACTCTCCAT
PPIA	NM_178320.2	F: GGTCCTGGCATCTTGTCCAT	96
		R: GCTTGCCATCCAACCACTCA
RPL4	NM_001014894.1	F: GCTCCCATTCGACCCGATATT	104
		R: AGCACTGGTTTGATGACCTGC
RPL32	NM_001034783.2	F: AGACCCCTCGTGAAGCCTAA	95
		R: CCGCCAGTTCCGCTTGATTT
SGLT1	NM_174606.2	F: TACATTAAGGCGGGGGTGGT	91
		R: AGGGACAGGACAGACAGGTA
YWAHZ	NM_174814.2	F: ACCTACTCCGGACACAGAACA	89
		R: TCATCATATCGCTCAGCCTGC

ISSN: 1297-9716