Figure 3
From: Ganjam virus/Nairobi sheep disease virus induces a pro-inflammatory response in infected sheep
Optimisation and validation of PCR primers for NSDV/GV detection and quantitation. A) Example gradient PCRs of F1/R1a and F2/R2 primer pairs. B) Example real-time PCR with 100-500nM primer concentration for F1/R1a primer pair. C) Sensitivity determination for real-time PCR with F1-R1a primer pair; serial dilutions were made of GV S segment DNA template from 4 to 4 × 107 copies per reaction and the real-time PCR carried out with F1/R1a. D) Plot of data from (C). E), F) Similar sensitivity determination and standard curve for primer pair F2/R2. G) Real-time PCR results for F1/R1a primer pair with 4 × 107 copies of GV (blue) or Dugbe virus (brown) S segment; purple and green lines are negative controls. H) As (G), but with F2/R2 primer pair.