Oligonucleotide | Restriction sites | Oligonucleotide sequence (5’-3’) |
---|---|---|
TetM1 | Pst I | GCCGCTGCAG AATTAAAAGTTAGTG |
TetM2 | Xba I | GAAATCTAGA TTATATAACAACTTAAATTAC |
TetM3 | Sbf I | CGATCCTGCAGG CAGAATTAAAAGTTAGTG |
TetM4 | Mlu I | CAGTACGCGT TTATATAACAACTTAAATTAC |
Tpn1 | Nde I/NcoI | GCGCATATGCCATGG AAAAAAAGGAATTTCGTG |
Tpn2 | Xho I | CGCCACTCGAG ATTATTCAACATAGTTCCCTTC |
P97-1 | Nde I | CGATCATATG ACGGGGATTTAAAACAGAAAC |
P97-2 | Nco I | GAATCCATGG CACCAACAATTCCGGCAGTC |
TetMF | N/A | GTGGACAAAGGTACAACGAG |
TetMR | N/A | CGGTAAAGTTCGTCACACAC |
Linker-A | N/A | CGACTGGACCTGGA |
Linker-B | N/A | GATAAGCAGGGATCGGAACCTCCAGGTCCAGTCG |
L-PCR-C | N/A | GATAAGCAGGGATCGGAACC |
L-PCR-L | N/A | GATAGGGTTGAGTGTTGTTC |
L-PCR-R | N/A | TAGTCGGATAGATAAAGTAC |