Figure 2From: Innate immune markers that distinguish red deer (Cervus elaphus) selected for resistant or susceptible genotypes for Johne’s diseaseCharacteristics of mature deer monocyte-derived macrophages. (A) Monocyte-derived macrophages stained positive (black) for α-naphthyl esterase at day 7 of culture (20X magnification). This enzyme is detected primarily in monocytes and macrophages and is virtually absent from granulocytes and other leukocytes, confirming that these adherent cells derived from deer blood are of a monocytic lineage. (B) Ziehl-Nielsen stain of deer MDM (blue nucleus) which has phagocytosed MAP bacilli; red, acid-fast microorganisms (100X magnification).Back to article page