Innate immune markers that distinguish red deer (Cervus elaphus) selected for resistant or susceptible genotypes for Johne’s disease

Table 1 Cervus elaphus primer sequences

Target	Primer sequence (5' to 3')	Amplicon size (bp)	Accession number
Β2M forward	GGCTGCTGTCGCTGTCT	75	DQ482731.1
Β2M reverse	TCTGGTGGGTGTCTTGAGTACA	75	DQ482731.1
PPIB forward	TGGCTACAAAGACAGCAAATTCC	148	NM_174152.2*
PPIB reverse	CCAGGCCCATAATGTTTAAGCT	148	NM_174152.2*
iNOS forward	GAAGAGGCTGAGAAGCAGAGGTT	98	NM_001076799.1*
iNOS reverse	TCCAGCACCTCCAGGAATGT	98	NM_001076799.1*
IL-1α forward	ATCCACGAGGAATGCATCCT	147	EU860100.1
IL-1α reverse	AGAATCCTCTTCTGATACATAAGCAACA	147	EU860100.1
TNF-α forward	AGGGAAGAGCAATCCCCAACT	123	U14683.1
TNF-α reverse	CTGAGCGTTGATGTTGGCTACA	123	U14683.1
IL-23p19 forward	GATGTCCCCCGTATCCAGTGT	114	EU860097.1
IL-23p19 reverse	CAGCAGCTTCTCGTAAAAAACCA	114	EU860097.1
IL-12p35 forward	GCCTCAACTACTCCCAAAACCT	83	U57751.1
IL-12p35 reverse	GCAGGAGTAAAATTCTAGGGTTTGTC	83	U57751.1
IL-10 forward	CGGTGGAGCAGGTGAAGAG	71	U11767.1
IL-10 reverse	AAACTCACTCATGGCTTTGTAGACA

* Accession number for corresponding bovine gene which was used to BLAST search the AgResearch Cervine Sequence Database to obtain cervine sequence from which to design primers.

ISSN: 1297-9716