Small ruminant macrophage polarization may play a pivotal role on lentiviral infection

Table 1 Primers used for amplification and cloning of the cytokines and the Caev-Cork env gene used in entry assays.

Molecule	Primer		Probe	Amplicon length
	Forward	Reverse
IL-4	AATTCTCGAG ACCATGG GTCTCACCTCCCAGC	AATTTGGTACC TCAACACTTTGAGTATTTCTCC		472 nt
IL-13	AATTCTCGAG ACCATGG CGCTCTTCTTGAC	ATTTGGTACC TCAGTTGTAACTTCCATTGCG		556 nt
IL-10	AATTCTCGAG ACCATG CCCAGCAGCTCAG	ATTTGGTACC TTACATCTTCGTTGTCATGTA		419 nt
CAEV-Cork env	ATATAGATCT CCACCATG	ATTTGCGGCCGC TATTAGTCCTCTTTAG		2834 nt
TNF-α qPCR	GGTGCCTCAGCCTCTTCTC	GAACCAGAGGCCTGTTGAAG	6-FAM-TGGTTGCAGGAGCCACCACG-TAMRA	134 nt
CD80 qPCR	CTGTGATTACAACACGACCACTGA	ATGGTGCGGTTCTCGTATTCA	6-FAM-AACTGGCAAGCCTTCGGATCTACTGGC-TAMRA	128 nt [30]
A3Z1 qPCR	TCCGTTCTTGGAATCTGGAC	GTATAGATGCGGGAGGCAAA		151 nt
MR qPCR	TGGCAAATCCAGTTGTTAAGATGTT	AGAATGTTGAATACTGTGGCGAGTT		91 nt [31]
DC-SIGN qPCR	GGTTCCGGAGTCTGACTGAAGTT	GGTCAGGCGCTGTAGGATCTC		73 nt
IL-10 qPCR	CGGCGCTGTCATCGTTTT	TCTTGGAGCATATTGAAGACTCTCTTC	6-FAM-CCTGCTCCACCGCCTTGCTCTTG-TAMRA	82 nt

Sequences are listed 5′to 3′; restriction enzyme recognition sites are underlined; Kozak sequences are in bold. A3Z1, MR and DC-SIGN were amplified using SybrGreen Master Mix (Takara).
Primers and probes (when corresponding) for relative expression quantification using real time PCR (qPCR) are also indicated.

ISSN: 1297-9716