Duck MDA5 functions in innate immunity against H5N1 highly pathogenic avian influenza virus infections

Table 1 Gene cloning PCR primers used in this study

Primer name	Sequence of Oligonucleotide (5′ → 3′)	Purpose
idMDA5-f	AGRSMTTACCARATGGAAGTKG	Gene cloning
idMDA5-r	AARTGTTCTGCACARACRCGWTC
idPKR-f	CACCTAATTTTGATAATGCAAGAAA	Gene cloning
idPKR-r	ATAAATGTCTACTTCCTTTCCATA
idOASL-f	TTCCTCAAGGAGCGCTGCTTC	Gene cloning
idOASL-r	GGGTCGGCGGGATCCAGGAT
5rdM5r-1	AATGAGATTTTCAGCTGAGAATCACCAC	5′-Race
5rdM5r-2	TGATCTTTGGTAATGTAAACAG
3rdM5f-1	GATCTCAGCCATATGAACAGTGGGTG	3′-Race
3rdM5f-2	TGATGATGATGATGAACCAGC
dMDA5-f	ATGTCGACGGAGTGCCGAGACG	Site mutation
dMDA5-site mutation-r	AGGTGCTCTCATCAGCACGAGCTCGAC
dMDA5-site mutation-f	GCCCGTGGTCGAGCTCGTGCTGATGAG	Site mutation
dMDA5-r	TCAGTCTTCATCACTTGAAGGACA
pCAGGS-MDA5-f	TAACTCGAG ACCATGTCGACGGAGTGCCGAGACG	MDA5 cloning
pCAGGS-MDA5-r	ATTGCTAGC TCACTTGTCATCGTCGTCCTTGTAGTCATCTTCATCACTTGA
pCAGGS-MDA5ΔRD-f	TAACTCGAG ACCATGTCGACGGAGTGCCGAGACG	MDA5-ΔRD cloning
pCAGGS-MDA5ΔRD-r	ATTGCTAGC TCACTTGTCATCGTCGTCCTTGTAGTCAGGGTTTTTCTTATATG
pCAGGS-MDA5ΔCARD-f	TAACTCGAG ACCATGACAGGAGGAAAAGAGAATAA	MDA5-ΔCARD cloning
pCAGGS-MDA5ΔCARD-r	ATTGCTAGC TCACTTGTCATCGTCGTCCTTGTAGTCATCTTCATCACTTGA
pCAGGS-MDA5ΔCARD + ΔRD-f	TAACTCGAG ACCATGACAGGAGGAAAAGAGAATAA	MDA5ΔCARD + ΔRD cloning
pCAGGS-MDA5ΔCARD + ΔRD-r	ATTGCTAGC TCACTTGTCATCGTCGTCCTTGTAGTCAGGGTTTTTCTTATATG
pCAGGS-MDA5-CARD -f	TAACTCGAG ACCATGTCGACGGAGTGCCGAGACG	MDA5-CARD cloning
pCAGGS-MDA5-CARD -r	ATTGCTAGC TCACTTGTCATCGTCGTCCTTGTAGTCATTTCCACTTAAATCAT
pCAGGS-DK212-NS1-f	TAACTCGAG ACCATGGATTCCAACACTGT	DK212-NS1 cloning
pCAGGS-DK212-NS1-r	ATTGCTAGC TCACTTGTCATCGTCGTCCTTGTAGTCAACTTTTGACTCAAT

Note: CGTCTC was the recognition site of restriction enzyme Xho I and GCTAGC was the recognition site of restriction enzyme Nhe I. R = A/G, S = C/G, M = A/C, K = G/T, W = A/T. f = forward primer; r = reverse primer.

ISSN: 1297-9716