Figure 3

Immunofluorescence and confocal microscopy of PRV infected RBC. (A) Fluorescent labeling of the PRV ơ1-protein (green) in ex vivo infected RBC. The nuclei were stained with Hoechst (blue). Non-infected cells are shown at 0 dpi, and infected cells are shown at 1, 7 14 and 21 dpi. Large PRV inclusions at 21 dpi (marked by arrowheads) can also be observed in phase contrast. (B) Confocal images of ex vivo infected RBC at 21 dpi, stained with anti-dsRNA (red) and anti-PRV-ơ1 (green). The nuclei were stained with Hoechst (blue). dsRNA was detected within PRV inclusions, and either partly (i) or completely (ii) co-localized with PRV ơ1. Scale bar 2 μm.