Recombinant proteins from Gallibacterium anatis induces partial protection against heterologous challenge in egg-laying hens

Table 2 Scoring of lesions in birds infected with Gallibacterium anatis

Organ	Description	Score	Protective response
Peritoneum	Normal	0	Yes
	Mild cloudiness	1
	Moderate cloudiness and serous exudates	2
	Moderate cloudiness and serous exudates with fibrin spots	3	No
	Complete cloudiness and local fibrinopurulent exudates	4
	Complete cloudiness and diffuse fibrinopurulent exudate	5
Ovary	Normal	0	Yes
	Mild vascular congestion. No deform follicles	1
	Moderate vascular congestion. Max. one deform follicle	2
	Complete vascular congestion and serous exudates with fibrin spots. Deformed follicles	3	No
	Complete vascular congestion and fibrinopurulent exudate	4
	Complete vascular congestion, fibrinopurulent exudate and deformed follicles	5
Oviduct	Normal	0	Yes
	Vascular congestion	1
	Vascular congestion, edema and serous exudates	2
	Vascular congestion, edema and local fibrinopurulent exudates	3	No
	Vascular congestion, edema and diffuse fibrinopurulent exudates	4
	Vascular congestion, edema, diffuse fibrinopurulent exudates and necrosis	5

48 h after challenge all birds were euthanized and scoring of lesions was done accordingly. Scores 0, 1 and 2 were regarded as inflammatory reaction without infection and considered as a protective response against infection. Scores 3, 4 and 5 was regarded as inflammation with infection and considered as no protection of the response, i.e., infection had overwhelmed the inflammatory response.

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