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Figure 5 | Veterinary Research

Figure 5

From: Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture

Figure 5

Replication of CEV in gill explant cultures and in primary fin cultures of various carp strains. Cultures were obtained from koi (Koi) and common carp from following strains: Amur wild carp (AS), Ropsha (Rop) Prerov (PS). Cultures were infected with CEV from the carp strain for 48 h at 15 or 25 °C. The CEV was re-isolated from KSD affected carp. As a surrogate for virus replication, the expression of mRNA encoding the viral core protein P4a (CEV P4a mRNA levels) is shown as mean (+SD) copy number of the P4a gene normalised against 100 000 copies of the carp 40S ribosomal protein S11 from n = 3 cultures.

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Veterinary Research

ISSN: 1297-9716