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Figure 4 | Veterinary Research

Figure 4

From: Surface displaying of swine IgG1 Fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction

Figure 4

Characterization and evaluation of BacMam expressing E2 based on swine IgG1 Fc surface display. A Schematic diagram of donor vectors of BacMam virus vaccines. B Western blot detection of pFc and E2 protein expression in insect cells infected with recombinant baculoviruses. BV-VSVG-ED-CMV-E2 or BV-VSVG-ED-pFc-CMV-S/P-E2-infected Sf-9 cells were harvested 2 days after infection and used for analysis. The E2 protein (55 kDa) was detected by rabbit anti-CSFV polyclonal antibody, and His6-tagged pFc (27 kDa) was analyzed by anti-His6 monoclonal antibody. Anti-β-actin was included as an internal control. C IFA detection of expression of E2 protein in BV-VSV-ED-CMV-E2 or BV-VSVG-ED-pFc-CMV-S/P-E2-transduced IEC cells. The cells were fixed at 48 h after transduction, and analyzed by rabbit anti-CSFV polyclonal antibody and Alexa 594-conjugated goat anti-rabbit IgG. Uninfected IEC cells were served as a negative control.

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Veterinary Research

ISSN: 1297-9716