Figure 3

Cytotoxic T lymphocyte responses to PPRV epitopes. In vitro peptide-stimulated PBMC obtained 17–21 days post-infection from PPRV (IC’89)-infected sheep were used as effector cells (E) in cytotoxicity assays. Autologous LPS-blast cells were used as target cells (T) and labeled with PKH67 cell linker dye. Cell cytotoxicity was measured by propidium iodide staining at different effector cells (E) to target cells (T) ratios (E:T). Gating on bright PKH67⁺ and propidium iodide⁺ events by flow cytometry was used to calculate the percentage of target cell lysis. A Specific lysis (mean ± SD) to F10, H4, H9, H10, NP8 and NP9 peptides in PPRV IC’89 infected sheep. Student’s t test (peptide vs no peptide); *p < 0.05. B Representative dot-plots showing dead target cells gating. C Specific lysis (mean ± SD) of unpulsed, peptide-, or PPRV IC’89-pulsed autologous target cells. One way ANOVA with Dunnett’s post-test (peptide or PPRV IC’89 vs no peptide); *p < 0.05.