Figure 5
Interaction between Nb84 and purified MOMP. To obtain the saturation binding curve, ELISA plates were coated with purified MOMP monomers (1 µg/mL) and the interaction with increasing concentrations of His-tagged Nb84 (1 × 10−6 to 1 × 102 µg/mL) was measured. A competition assay was performed to assess the inhibition of the interaction of His-tagged Nb84 with MOMP by increasing amounts of strep-tagged Nb84. His-tagged Nb84 (5 × 10−2 µg/mL) and a serial dilution of strep-tagged Nb84 (1 × 10−6 to 1 × 102 µg/mL) were added to ELISA plates coated with MOMP (1 µg/mL). The ELISA was developed using a mouse anti-Histidine tag monoclonal antibody and goat anti-mouse IgG conjugated to alkaline phosphatase. The error bars represent the standard deviations.