Figure 2

Bovine enteroids cultivated in the presence of Rho kinase, p38 MAP kinase and TGFβR inhibitors. A Representative images showing the growth and development of an individual enteroid cultivated in the presence of Rho kinase, p38 MAP kinase and TGFβR inhibitors. Arrows, crypt buds. B Representative images showing the development of enteroids following one (P1), two (P2) or four (P4) rounds of serial passage and culture. C Representative images showing the development of cryopreserved enteroids following resuscitation and 11 days of culture, and after subsequent rounds of serial passage.