A Mycobacterium avium subsp. paratuberculosis relA deletion mutant and a 35 kDa major membrane protein elicit development of cytotoxic T lymphocytes with ability to kill intracellular bacteria

Table 1 MAbs used in the present study

mAb	Isotype	Specificity/source	Fluorochrome	Labeling
ILA11A	IgG2a	CD4 WSUMAC	PE CY5.5	Indirect
CACT138A	IgG1	CD4 WSUMAC	Alexa Fluor^® 647	Indirect
7C2B	IgG2a	CD8 WSUMAC	PE CY5.5	Indirect
CACT80C	IgG1	CD8 WSUMAC	Alexa Fluor^® 647	Indirect
ILA116A	IgG3	CD45R0 WSUMAC	Alexa Fluor^® 488; PE	Indirect
CAM36A	IgG1	CD14 WSUMAC	Alexa Fluor^® 647	Indirect
209MD26A	IgG2a	CD209 WSUMAC	PE CY5.5	Indirect
δG9	IgG2b	Perforin Biolegend	PE	Direct
B-D48	IgG1	Perforin Biolegend	PE/CY7	Direct
351927	IgG2a	Granzyme B R&D Systems	PE	Direct

ISSN: 1297-9716