Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species

Table 1 Primer and probe sequences for Treponema species-specific fourplex qPCR and conventional PCR

Species	Forward primer (sense)	Probe (sense)	Reverse Primer (antisense)	Size (bp)
T. denticola ^a	GGAAACTTAGGAATTCGATATGTAG	AGCATACAGCGATTATAACAAAGCCCTCGA	CCTTCTTTAGTTTCTTTGTGAGG	113
T. medium ^a	AAAGCGCTACGAATCCTAAG	TGCACCCTTGTTTACTACTGCACAGCC	ATCATTACCCGTCCACAAAG	119
T. phagedenis ^a	CCCGCAGGAAGGTATAATC	AATCCGCCTACGACTGCGATACCA	CACAGCTGTTGTGGTATTAAG	90
T. pedis ^a	ACACCGATTGTACTGAATGA	ACTACACGTGGAGTACCGAATGCT	CCACGAGCTTTCTACAGATT	118
T. denticola ^b	AGGAATGGCCTTTGAACCCGCA		CCGATGAACCCGTATCTTCACCGA	458
T. medium ^b	GGAACAGGCAGCCGCATTGGAT		CCGCCCATGTGAGGCTTGTGAT	515
T. phagedenis ^b	TCCGCCTACGACTGCGATACCA		CGGAACTGTCACAACTGGCGGA	785
T. pedis ^b	TGGATGTTACGGAAGAGACACCGA		TGCCCCACTCTTACAAGTTCATCCCA	295

ISSN: 1297-9716