Figure 5
From: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2
Zmp is partially implicated in mucin 16 (MUC16) ectodomain cleavage by S. suis. Relative densitometry of HeLa cell culture medium conditioned with non-concentrated S. pneumoniae supernatant, Zmp purified recombinant protein, S. suis P1/7 (WT) or S. suis Δzmp supernatant (3X) supplemented or not with 100 µg of recombinant Zmp protein. Samples were blotted on a nitrocellulose membrane and incubated with anti-human CA125 antibodies. Compared to the untreated cells, S. suis WT strain P1/7 supernatant cleaved MUC16 ectodomains. This activity was significantly reduced when using the supernatant of the Δzmp strain (p = 0.017). Supernatant of the compΔzmp significantly increased cleavage when compared to the Δzmp strain. Addition of 100 µg of Zmp protein to the supernatant of the Δzmp strain also restored WT phenotype activity. Data represent the mean ± SEM from at least three independent experiments. *(p < 0.05) indicates a significant difference between treated groups and the medium; #(p < 0.05) between treatment groups.