Table 1 Bacterial strains and plasmids used in this study
From: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2
Strains/plasmids | General characteristics | Source/references |
|---|---|---|
Escherichia coli | ||
TOP 10 | F− mrcA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
BL21 | F−ompT hsdSB (r −B , m −B ) gal dcm rne131 (DE3) | Invitrogen |
Streptococcus suis | ||
P1/7 | Wild-type, virulent European serotype 2 ST1 strain isolated from pig with meningitis | [56] |
Δzmp | Isogenic mutant strain derived from P1/7; in frame deletion of zmp gene | This work |
compΔzmp | Mutant Δzmp complemented with pMXzmp complementation vector | This work |
SC84 | Highly virulent clonal serotype 2 ST7 strain isolated from the 2005 human outbreak in China | [57] |
Streptococcus pneumoniae | ||
TIGR4 | Virulent serotype 4 strain isolated from human blood in Norway | ATCC |
Plasmids | ||
PCR2.1 | Apr, Kmr, oriR(f1) MCS oriR (ColE1) | Invitrogen |
pSET4s | Thermosensitive vector for allelic replacement in S. suis. Replication functions of pG + host3, MCS oriR pUC19 lacZ SpR | [20] |
pMX1 | Replication functions of pSSU1, MCS pUC19 lacZ SpR, malX promoter of S. suis, derivative of pSET2 | |
pET101 | Apr, pBR322 ori, T7 promotor | Invitrogen |
p4Δzmp | pSET4 s carrying the construct of zmp for allelic replacement | This work |
pMXzmp | pMX1 complementation vector carrying intact zmp gene | This work |
pET101zmp | pET101 carrying zmp gene for protein production | This work |