Table 1 List of primers used for qRT-PCR

1

TLR2-A

Forward 5′-TCGCTCCAACACCTTCGCATTC

Reverse 5′-GATTGTCACCGTCGATCCTCAGC

2

TLR2-B

Forward 5′-TCCTCATCCTGGTGGTCGTTGG

Reverse 5′-GGCTTCCGCTTGGCTTGGAG

3

TLR4

Forward 5′-TTCGGTTGGTGGACCTGAATCTTG

Reverse 5′-ACAGCTTCTCAGCAGGCAATTCC

4

NLRP3

Forward 5′-GCTCCTTGCGTGCTCTAAGACC

Reverse 5′-TTGTGCTTCCAGATGCCGTCAG

5

IL-10

Forward 5′-CAGCACCAGTCATCAGCAGAGC

Reverse 5′-GCAGGTGAAGAAGCGGTGACAG

6

IL-18

Forward 5′-AGATGATGAGCTGGAATGCGATGC

Reverse 5′-ATCTGGACGAACCACAAGCAACTG

7

PTGE

Forward 5′-GCCTTCTACAGCACGATCCTGATC

Reverse 5′-GCCTTCTTCCTGAGCCTCACTTG

8

ALOX5

Forward 5′-GCGGTTCACAATAGCCATCAACAC

Reverse 5′-GCTGTAGGTCAGGTCCTTCATTGC

9

ALOX15B

Forward 5′-GTGAAGGAGCGGACAGTGAAGTG

Reverse 5′-AACCAGGCATCCTCCAGGAAGAG

10

LOXHD1

Forward 5′-CACAGACAAGACCTTCCGCTTCC

Reverse 5′-GCAGTCCGTTCCTTCAGTTCCAG

11

IFN-γ

Forward 5′-TTCCTGATGGCGTGAAGAAGGTG

Reverse 5′-TCGGAGGATCCACCAGCTTCTG

12

GSDMA

Forward 5′-AGCCTCACAGAAGCCATCTCCTAC

Reverse 5′-GCTGCTGCTGCTCGCTGAAG

13

GSDME

Forward 5′-GCTGCGTGCCTGCTCTGATC

Reverse 5′-GCTCAGTGCCAAGGTGCCATC

14

TRAF2

Forward 5′-CGTGGTGATGAAAGGACCCA

Reverse 5′-AATGATGTGCTCCCGGTTGT

15

TNF-R1

Forward 5′-CCTGTCTGTCTTCCCTGTCC

Reverse 5′-GGTGCATGGGGTCTTTTCTA

16

TRADD

Forward 5′-CTAGAGCCCAAAGGAAGTCGAT

Reverse 5′-TGGCTGCTTCTCTGTGACAT

17

FADD

Forward 5′-GGGGTAAAGAGGCTGAACTCTTA

Reverse 5′-TGAGTCCTATTGCACTGCTGTC

18

Nrf2

Forward 5′-GATGTCACCCTGCCCTTAGA-3′

Reverse 5′-TCGTTCCATTTGTTCCTTCTG-3′

19

GPX-1

Forward 5′-AAGTGCTGCTGGTGGTCAACG

Reverse 5′-GTTGGTGGCGTTCTCCTGGTG

20

GPX-3

Forward 5′-GTGGCAGAGGAGTTCGGCAAC

Reverse 5′-TCTTGACAGTGGCGATGTTGGC

21

PRDX6

Forward 5′-GCATCCGCTTCCACGACTTCC

Reverse 5′-GGCGTTGATGTCCTTGCTCCAG

22

MAP1LC3

Forward 5′-GCTGCCAGTGCTGGACAAGAC

Reverse 5′-TCCTCATCCTTCTCCTGCTCGTAG

23

Beclin-1

Forward 5′-ACCGCAAGATTGTGGCTGAAGAC

Reverse 5′-TGAGCATAACGCATCTGGTTCTCC

24

mTOR

Forward 5′-AACCACTGCTCGCCACAATGC

Reverse 5′-CATAGGATCGCCACACGGATTAGC

25

HO-1

Forward 5′-TCATTGGCAAGAAGCATCCAGAGC-3′

Reverse 5′-GAACTTGGTGGCGTTGGAGACTC-3′

26

NF-κB

Forward 5′-CACATGGTGGTGACCGCCAATAG-3′

Reverse 5′-GTGCCATCGTATGTAGTGCTGTCC-3′

27

TNF-α

Forward 5′-TGATCGTGACACGTCTCTGC-3′

Reverse 5′-CAACCAGCTATGCACCCCAG-3′

28

IL-6

Forward 5′-TTCACCGTGTGCGAGAACAGC-3′

Reverse 5′-CAGCCGTCCTCCTCCGTCAC-3′

29

IL-1β

Forward 5′-AGCAGCCTCAGCGAAGAGACC-3′

Reverse 5′-GTCCACTGTGGTGTGCTCAGAATC-3′

30

Bax

Forward 5′-ACTCTGCTGCTGCTCTCCTCTC-3′

Reverse 5′-ATCCACGCAGTGCCAGATGTAATC-3′

31

Caspase-3

Forward 5′-TACCGGACTGTCATCTCGTTCAGG-3′

Reverse 5′-ACTGCTTCGCTTGCTGTGATCTTC-3′

32

Caspase-8

Forward 5′-GGAAGCAGTGCCAGAACTCAGAAG-3′

Reverse 5′-TTGTTGTGGTCCATGCACCGATAG-3′

33

Caspase-9

Forward 5′-CCGAAGGAGCAAGCACGACAG-3′

Reverse 5′-CATCTAGCATGTCAGCCAGGTCAC-3′

34

P53

Forward 5′-GGAGATGGAACCATTGCTGGAACC-3′

Reverse 5′-GCTCCTGCCAGTTGCTGTGATC-3′

35

Bcl2

Forward 5′-GAGTTCGGCGGCGTGATGTG-3′

Reverse 5′-TTCAGGTACTCGGTCATCCAGGTG-3′

36

Cytochrome C

Forward 5′-CCTAATCGCCGTGGCCTTCTTAAC-3′

Reverse 5′-GGAGGAGGTAGATGGTCGGATTGG-3′

37

NQO1

Forward 5′-TCGCCGAGCAGAAGAAGATTGAAG-3′

Reverse 5′-GGTGGTGAGTGACAGCATGGC-3′

38

GSTA2

Forward 5′-GGAGTCAATCCGGTGGCTGTTAG-3′

Reverse 5′-GGCTCTGCTCTGCACCATCTTC-3′

39

ATG5

Forward 5′-GGACGCATACCAACCTGCTT

Reverse 5′-TGCCATTTCAGTGGCGTACC

40

Dynein

Forward 5′-CGTTGCCAGCGTTACACCTATCC

Reverse 5′-GCCAGGACTGCCACCAACAC

41

F-actin

Forward 5′-ACCTGGATTGGAGAGGATGTCAGC

Reverse 5′-CGGCCTTCTTCAGCTCGTTCTTG

42

α-Tubulin

Forward 5′-GCGGCACGGCAAGTACATGG

Reverse 5′-CTTGGTCTTGATGGTGGCGATGG

43

TGFβ1

Forward 5′-GCCGACACGCAGTACACCAAG

Reverse 5′-GCAGGCACGGACCACCATATTG

44

β-actin

Forward 5′-CAACACAGTGCTGTCTGGTGGTAC-3′

Reverse 5′-CTCCTGCTTGCTGATCCACATCTG-3′