Figure 4

Frequencies of splenic IL-13 mRNA⁺ cells following stimulation with PMA/ionomycin or H. meleagridis. A, B Representative pseudocolor plots show IL-13 mRNA versus CD4 expression in pre-gated (not depicted) total live splenocytes isolated from birds 2 weeks pi and 5 weeks pi. Approximately 320 000 lymphocytes are shown in each plot and numbers indicate frequencies of IL-13 mRNA⁺ cells within total live splenocytes. Graphs on the right display frequencies of IL-13 mRNA⁺ live lymphocytes from all birds. Each symbol represents one bird, black and red colored symbols represent birds sacrificed 2 weeks pi and 5 weeks pi, respectively. A Scatter plots show percent of IL-13 mRNA⁺ cells within live lymphocytes after PMA/ionomycin stimulation compared to medium in control and infected birds (left panel). Right panel: comparison of IL-13 mRNA⁺ lymphocyte frequencies after stimulation with PMA/ionomycin between infected and control birds. B Scatter plots as in A but after H. meleagridis/E. coli stimulation and E. coli-only stimulation. Right panel shows in addition percent of IL-13 mRNA⁺ cells after E. coli correction for infected and control birds. Asterisks indicate p-value: *p ≤ 0.05.