| | Days post-infection | Animal number | Cecum | Liver |
|---|
LSa | IHCb | LS | IHC |
|---|
Control | 14 | 1 | 0 | − | 0 | − |
15 | 2 | 0 | − | 0 | − |
16 | 3 | 0 | − | 0 | − |
37 | 4 | 0 | − | 0 | − |
38 | 5 | 0 | − | 0 | − |
39 | 6 | 0 | − | 0 | − |
Infected | 14 | 7 | 3 | + | 0 | − |
15 | 8 | 4 | − | 0 | − |
16 | 9 | 2 | + | 0 | − |
37 | 10 | 2 | + | 0 | − |
38 | 11 | 2 | + | 0 | − |
39 | 12 | 2 | + | 2 | − |
- aLesion scoring (LS) system from 0 to 4 was applied; Cecum: 0 = no pathological changes; 1 = sporadic inflammation and/or mild thickening of the wall of one cecum; 2 = sporadic inflammation and/or mild thickening of the wall of both ceca; 3 = inflammation of both ceca and thickening of the intestinal wall with liquid fibrin or sporadic fibrinous coagula in the lumen. If only one cecum was affected, then lesion score 2 was applied; 4 = severe inflammation and necrosis in both ceca with compact fibrinous masses in the lumen of the ceca. If only one cecum was affected, then lesion score 3 was applied. Liver: 0 = no pathological changes; 1 = a few single punctiform necrosis up to 1 mm; 2 = single punctiform necrosis disseminated throughout the organ up to 1 mm or a few single punctiform necrosis more than 1 mm; 3 = single punctiform necrosis, disseminated throughout the organ more than 1 mm or some large area necrosis; 4 = confluent necrosis throughout the organ.
- bDetection of the parasite in cecum and liver was performed by immunohistochemistry.
