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Table 1 Monoclonal antibodies used for immunolabelling of whole blood for leukocyte counts

From: Immune responses upon experimental Erysipelothrix rhusiopathiae infection of naïve and vaccinated chickens

Abbreviation

Clone

Specificity

Fluorochrome

KUL01-RPE

KUL01a

Chicken mannose receptor MRC1L-B [44]

R-phycoerythrinc

CD41/61-Fitc

11C3b

Chicken CD41/61 intergrin (GPIIb-IIIa)

Fluoresceinc

CD45-PerCp/Cy5.5

UM16-6b

Chicken CD45, all isoforms [41]

Peridinin chlorophyll-cyanine 5.5d

  1. aPurchased from Southern Biotech.
  2. bPurchased from Bio-Rad Antibodies.
  3. cFluorochrome conjugated by manufacturer.
  4. dFluorochrome conjugated using Lightning-Link™ conjugation kits (Expedeon) according to the manufacturer’s protocol.