Figure 15

The larval molting was inhibited by siRNA-279 in IIL stage. In the 12 h IIL stage, the larval molting was obviously suppressed by inhibitor NaF, no obvious separation between the old and the new cuticle was observed except the larval tail end, while the separation of new and old cuticles of 12 h IIL in the other three groups was clearly observed. In the 24 h IIL stage, the obvious suppression of larval molting was also observed in siRNA group due to the successful inhibition of TsPPase expression and activity. The area in the red box was enlarged for observation.