Strain or plasmid | Characteristics | References |
---|---|---|
Streptococcus suis | ||
 P1/7 | Virulent serotype 2 ST1 strain isolated from a case of pig meningitis in the United Kingdom | [23] |
 5P1/7Δlmb | Isogenic mutant derived from P1/7; in frame deletion of lmb gene | [18] |
 P1/7 Δlmb/ΔcpsG | Isogenic mutant derived from P1/7; in frame deletion of lmb and cpsG gene | This study |
 P1/7 ΔcpsF | Isogenic mutant derived from P1/7; in frame deletion of cpsF | [21] |
 P1/7 comp Δlmb | Mutant Δlmb complemented with pMX1-lmb complementation vector | This study |
Escherichia coli | ||
 TOP10 | F− mrcA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu) 7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
 MC1061 | Host for pMX1 derivatives | [24] |
Plasmids | ||
 pCR2.1 | Apr, Kmr, pUC ori, lacZΔM15 | Invitrogen |
 pSET4s | Spcr, pUC ori, thermosensitive pG + host3 ori, lacZΔM15 | [25] |
 pMX1 | Replication functions of pSSU1, MCS pUC19 lacZ SpR, malX promoter of S. suis, derivative of pSET2 | |
 p4ΔcpsG | pSET-4 s carrying the construct for lmb allelic replacement | This study |
 pMX1-lmb | pMX1 carrying intact lmb gene | This study |