Figure 12

Western blotting analysis of PPARγ antagonist abrogating the rTsDPP1-induced M2 polarization. Mouse peritoneal macrophages were pretreated with a PPARγ antagonist (GW9662) and cultured with rTsDPP1 at 37 °C and 5% CO₂ for 24 h. The soluble cell proteins from treated peritoneal macrophages were separated by SDS‒PAGE and analysed by Western blotting. A Western blotting of M2 polarization markers and pathways (p-STAT6, STAT6, PPARγ and Arg1). Tubulin served as an internal control. B The intensity of p-STAT6, STAT6, PPARγ and Arg1 protein relative to the intensity of tubulin. *P < 0.05 compared to the PBS group, ^#P < 0.05 between two groups.