Figure 13

NO production in the supernatant of cultured RAW264.7 macrophages. A The standard curve of NO concentration. B Macrophages were cultured with only rTsDPP1, AW ESA, TRX, IL-4 or LPS, or after being precultured with LPS, macrophages were recultured with different proteins (rTsDPP1, AW ESA and IL-4). C Macrophages were preincubated with LPS for 24 h, cultured with a STAT6 inhibitor (AS1517499, 100 nM) or a PPARγ antagonist (GW9662, 10μM) for 24 h, and finally incubated with rTsDPP1, AW ESA and IL-4 for 24 h,. LPS (200 ng/mL) was used as a positive control, while IL-4 (20 ng/mL) was used as a negative control. *P < 0.05 compared to the PBS group, ^#P < 0.05 compared to the LPS group.