Figure 2

IFT and confocal microscopy of rTsDPP1 binding to RAW264.7 macrophages. RAW264.7 macrophages were cultured with diverse proteins at 37 °C for 2 h. Different sera (1:10; anti-rTsDPP1 serum, infection serum and preimmune serum) served as primary antibodies, and Alexa Fluor 488-conjugated anti-mouse IgG (1:100) was used as the secondary antibody. Cell nuclei were stained with DAPI. A Specific binding of rTsDPP1 to macrophages was observed under a fluorescence microscope. B The binding site of rTsDPP1 with macrophages was localized on the cell membrane by confocal microscopy. Scale bars: 25 μm.