Table 1 Specific primer sequences of macrophage markers and cytokines for qPCR analysis
Gene | Forwards (5′-3′) | Reverse (5′-3′) |
|---|---|---|
GAPDH | GGTTGTCTCCTGCGACTTCA | TGGTCCAGGGTTTCTTACTCC |
iNOS | TGGAGCCAGTTGTGGATTGTC | GGTCGTAATGTCCAGGAAGTAG |
TNF-α | GGTGCCTATGTCTCAGCCTCTT | GCCATAGAACTGATGAGAGGGAG |
IL-1β | TGGACCTTCCAGGATGAGGACA | GTTCATCTCGGAGCCTGTAGTG |
IL-6 | TACCACTTCACAAGTCGGAGGC | CTGCAAGTGCATCATCGTTGTTC |
TGF-β | TGATACGCCTGAGTGGCTGTCT | CACAAGAGCAGTGAGCGCTGAA |
IL-10 | CGGGAAGACAATAACTGCACCC | CGGTTAGCAGTATGTTGTCCAGC |
CD206 | GTTCACCTGGAGTGATGGTTCTC | AGGACATGCCAGGGTCACCTTT |
Arg1 | CATTGGCTTGCGAGACGTAGAC | GCTGAAGGTCTCTTCCATCACC |
CD64 | ACCTGAGTCACAGCGGCATCTA | TGACACGGATGCTCTCAGCACT |