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Fig. 5 | Veterinary Research

Fig. 5

From: luxS contributes to intramacrophage survival of Streptococcus agalactiae by positively affecting the expression of fruRKI operon

Fig. 5

The in vitro binding of CcpA to the fruRKI promoter or luxS promoter. A Diagrams depicting the noncoding region of the fruRKI operon. The magnified region indicates the promoter sequences, including the putative cre for CcpA binding (underlined), and the −10 and −35 positions are highlighted and labeled. B Diagrams depicting the noncoding region of the luxS gene. The putative cre is underlined, and the −10 and −35 positions are highlighted and labeled. C An electrophoretic mobility shift assay (EMSA) showing the binding ability of CcpA protein to the fruRKI or luxS promoters (25 nM). The concentrations of CcpA ranged from 0 to 1.0 μM.

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Veterinary Research

ISSN: 1297-9716