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Table 1 Binding affinity of anti-PD-1 mAbs to BoPD-1-His protein

From: Development of a high-affinity anti-bovine PD-1 rabbit–bovine chimeric antibody using an efficient selection and large production system

Anti-PD-1 mAb

ka (1/Ms)

kd (1/s)

KD (M)

5D2**

1.84 × 104 ± 0.27

2.15 × 10–4 ± 0.44

1.22 × 10–8 ± 0.39

1D10F1

1.63 × 106 ± 0.16*

1.80 × 10–3 ± 0.06*

1.11 × 10–9 ± 0.07*

4F5F2

7.39 × 105 ± 0.90*

2.08 × 10–3 ± 0.28*

2.85 × 10–9 ± 0.50*

  1. These values were measured on a Biacore X100 instrument
  2. *p < 0.05 (vs. 5D2; Tukey’s test)
  3. **The values for 5D2 were shown in a previous paper [10]
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Veterinary Research

ISSN: 1297-9716