Figure 2

rHcL6 binding with goat Th9 cells. Goat PBMCs were incubated with rHcL6 for 2 h, and then Th9 cells were sorted by flow-sorting cytometry. Th9 cells treated with (A) or without (C) rHcL6 protein were incubated with rat anti-rHcL6 IgG as the primary antibody. Th9 cells pretreated with rHcL6 were incubated with normal rat IgG as the primary antibody (B). DAPI (blue) and Cy3-conjugated secondary antibodies (red) were utilized for double staining. Merge, overlap of Cy3 and DAPI channels. Scale bars, 10 μm.