Figure 3

rHcL6 induces a goat Th9 immune response. Goat PBMCs were stimulated with different concentrations of rHcL6 (0, 5, 10, 20, 40 and 60 μg/mL). The significance level was set at ***p < 0.001, ****p < 0.0001, and “ns” as nonsignificant compared with the control (blank, 0 μg/mL). Data are representative of three independent experiments. A: Relative fold change in IL-9 transcription. B: IL-9 secretion profiles. The secretion of IL-9 in the culture supernatant was determined by ELISA. C: The effects of rHcL6 on Th9 cell proliferation. Th9 cells were detected by flow cytometry using typical intracellular cytokine antibodies (IL-9 and IL-10). D: Proportion of the Th9 cell population with different concentrations of rHcL6.